Methods

Methods Overview

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Integrative

  1. Measurement of pancreatic secretion in vivo in small animal models
  2. Measurement of pancreatic protein synthesis Maria Dolors Sans Gili, University of Michigan
  3. The isolated perfused pancreas
  4. Preparation of pancreatic fragments for studying the role of nerves and islets on pancreatic exocrine secretion Gino Saccone and Savio G. Barreto, Flinders Univ, Australia
  5. Identification and quantification of neural pathways to the pancreas
  6. An isolated rat pancreas preparation for studying pancreatic spinal mechanosensitive and chemosensitive afferent activity Ann C. Schloithe, C.M. Woods and Gino Saccone, Flinders Univ, Australia
  7. Measurement of cholecystokinin Rodger Liddle, Duke University
  8. Quantitation of pancreatic blood flow by microspheres 
  9. Assessment of pancreatic microcirculation by intravital microscopy 
  10. Quantitating pancreatic growth in response to diet and hormones Stephen Crozier and John Williams, University of Michigan
  11. Quantitation of autophagy in the pancreas at the tissue and cell levels Juan Iovanna and Maria Ines Vaccaro, Marseille France and Buenos Aires Argentina
  12. Isolation of Pancreatic RNA Robert C. De Lisle, University of Kansas
  13. Incorporation of polaxamer during tissue dissociation allows isolation of high quality RNA from FACS-sorted pancreatic cells Janivette Alsina, Steven Leach and Jennifer Bailey, Johns Hopkins University
  14. Transgenic expression of genes in the pancreas
  15. Cre-Lox excision to selectively delete or activate genes in the pancreas
  16. Lineage tracing in cells of the exocrine pancreas - Andy Rhim, University of Michigan
  17. Evaluating the pancreas and pancreatic function in mouse models
  18. Zebrafish as a model organism for studying pancreatic development and function

Acinar Cell Studies

  1. Isolation of rodent pancreatic acinar cells and acini by collagenase digestion John Williams, University of Michigan
  2. Isolation of human pancreatic acinar cells from surgical tissue samples Matthew C. Cane1, Robert Sutton2 and David N. Criddle1, 1University of Liverpool and 2Royal Liverpool University Hospital
  3. Radiolabeled binding studies of hormones and growth factors to acini
  4. Photoaffinity labeling of pancreatic receptors Maoqing Dong and Laurence J Miller, Mayo Scottsdale
  5. Measurement of intracellular calcium concentration in pancreatic acini Matthew J. Betzenhauser*, Jong Hak Wan, Hyungseo Park # and David Yule, *Columbia University Medical School, University of Rochester, #Konyang University
  6. Visualising the endoplasmic reticulum and its contacts with other organelles in live acinar cells   Hayley Dingsdale, Lee P Haynes, Alexei Tepikin and Gyorgy Lur, University of Liverpool
  7. Measuring Ca2+ dynamics in pancreatic acini using confocal microscopy Arbahim I. Orabi(1), Michael H. Nathanson(2) and Sohail Z. Husain(1), (1) University of Pittsburg, (2)Yale University
  8. Characterizing ion channel in acinar cell by patch clamp electrophysiology 
  9. Amino acid and glucose transport in isolated acini
  10. Permeabilized acinar cells as a tool to study the effects of membrane impermeant agents on acinar exocytosis Diana DH Thomas and Guy E Groblewski, University of Wisconsin
  11. Knockdown of gene expression in isolated acini by siRNA and shRNA
  12. Expression of exogenous proteins in acini with adenoviral vectors
  13. Purification of pancreatic zymogen granules using percoll gradients Xuequn Chen and John A. Williams, University of Michigan
  14. Isolation, purification and protein content of pancreatic endoplasmic reticulum John A. Williams and Xuequn Chen, University of Michigan and Wayne State University
  15. Isolation of pancreatic mitochondria and measurement of their functional parameters Irina V. Odinokova (1,2), Natalia Shalbuyeva (1), Anna S. Gukovskaya(1) and Olga A. Mareninova (1), (1)UCLA and (2)Russian Academy of Sciences
  16. Isolation of pancreatic plasma membranes
  17. Isolation and characterization of pancreatic lysosomes/endosomes Fred S Gorelick, Yale Univesity
  18. Chromatin Immunoprecipitation (ChIP) from pancreatic acinar cells and whole pancreatic tissue  Elena Fazio, Rashid Mehmood and Christopher Pin, University of Western Ontario
  19. Quantitation and visualization of protease activation in pancreatic acini Burkhard Kruger and Markus Lerch, University of Rostock, Germany, and Ernst-Moritz-Amdt University, Germany
  20. Use of mass spectrometry to identify proteins in acinar cells and organelles Xuequn Chen, Wayne State University
  21. Studies of protein phosphorylation in isolated acini
  22. Visualizing the actin cytoskeleton with fluorescent phalloidin
  23. Quantitating activation of small G proteins in Ras and Rho families by pull down assays 
  24. Visualization of exocytosis in pancreatic acinar cells by fluorescence microscopy Yujin Jang and Peter Thorn, University of Queensland
  25. Immunofluorescent Surface Labeling of Externalized Pancreatic Zymogen Granules or Endolysosomal Vesicles Following Exocytosis Diana D.H Thomas and Guy E. Groblewski, University of Wisconsin

Duct Cell Studies

  1. Microperfusion and micropuncture analysis of ductal secretion Hiroshi Ishiguro, Martin Seward, and Akiko Yamamoto, Nagoya University and Manchester University
  2. Optical measurement of ductal fluid secretion M. Pilar Hernández-Lorenzo(1), Mónica García(1), José Julián Calvo(1), Martin C. Steward(2) and Jose San Roman(1), (1)University of Salamanca and (2)University of Manchester
  3. Monitoring of intracellular pH and H+ fluxes in duct cells 
  4. The use of siRNA to alter protein expression in duct fragments Dongki Yang and Shmeul Muallem
  5. Characterizing the protein components of pancreatic juice
  6. Characterizing ion channels in duct cells by patch clamp electrophysiology 
  7. Computational modeling of pancreatic electrolyte transport 
  8. Ductal derived cell lines for use in studying duct function and cystic fibrosis

Centroacinar Cell Studies

  1. Isolation of Centroacinar Cells Steve Leach, Johns Hopkins University

Stellate Cell Studies

  1. Isolation of quiescent pancreatic stellate cells from rat and human pancreas Minoti Apte, University of New South Wales
  2. Assesment of stellate cell growth (proliferation) and migration Minote Apte, University of New South Wales
  3. Highly efficient siRNA delivery into primary cultures of rat pancreatic stellate cells Phoebe Phillips, University of New South Wales
  4. Analysis of senescence markers in rodent pancreatic stellate cells Sarah Müller, Brit Fitzner, and Robert Jaster, University Medicine Rostock
  5. Quantitating fibrosis and stellate cell activation in vivo
  6. Contribution of bone marrow derived mesenchymal stem cells to stellate cell populations in pancreatic disease

Digestive Enzymes and Digestion

Cell Lines and Primary Cell Cultures

  1. Culture of pancreatic acinar cells on a extracellular matrix
  2. Three dimensional culture of pancreatic acini
  3. Monolayer cultures of dog pancreatic duct cells as a model for pancreatic duct function Toan Nguyen, Thomas Wong, Christopher Savard and Sum P. Lee, University of Tennessee and Memphis VA Medical Center, University of Washington, Hong Kong University
  4. Culture of pancreatic AR42J cell for use as a model for acinar cell function Antonio Gonzalez, Patricia Santofimia-Castaño and Gines M. Salido, University of Extremadura, Spain
  5. Pancreatic cell lines derived from human pancreatic tumors

Pancreatic Disease Models

  1. Secretagogue (Caerulein) induced pancreatitis in rodents Julia Mayerle, Matthias Sendler, and Markus M. Lerch, Univ of Griefswald
  2. L-arginine-induced experimental acute pancreatitis Rajinder Dawra and Ashok Saluja, University of Minnesota
  3. Choline deficiency as a model for hemorrhagic pancreatitis
  4. Ethionine induced pancreatitis and pancreatic atrophy
  5. Use of coxsackievirus B3 as model of acute pancreatitis Jonathan H. Hall and Dahn L. Clemens
  6. Retrograde infusion of bile acids into the pancreatic duct to induce pancreatitis in rats and mice George Perides, Gijs JD van Acker, Johanna Laukkarinen and Michael Steer, Tufts Medical Center
  7. Modeling alcoholic pancreatitis by ethanol feeding and lipopolysaccharide (LPS) challenge Alain Vonlaufen, University of Geneva
  8. Pancreatic duct ligation models of pancreatitis
  9. Quantitating inflammation in a mouse model of acute pancreatitis, Madhav Bhatia and  Hon Yen Lau, University of Otago, Christchurch
  10. Ras mutations and the modeling of pancreatic cancer
  11. Xenograft models of pancreatic cancer cell growth
  12. Tumor-stromal interactions assessed by co-injection of pancreatic stellate and cancer cells Rosa Hwang, M.D. Anderson

Human Studies

  1. The CCK – Secretin test of pancreatic secretion 
  2. Other pancreatic function tests
  3. Visualizing the pancreas by imaging techniques
  4. Collection and analysis of human pancreatic juice for biomarkers and gene mutations
  5. Fecal measurements of elastase as evaluators of pancreatic function
  6. Genotype analysis for pancreatic disease
  7. Evaluation of pancreatic cyst fluid
  8. Histological evaluation of the pancreas in the diagnosis and characterization of pancreatic disease
  9. Proteomic analysis (GeLC-MS/MS) of endoscopically (ePFT) collected pancreatic fluid Joao A Paulo, Peter A Banks, Hanno Steen and Darwin Conwell, Children's Hospital Boston and Harvard Medical School