Methods

Methods Overview

This section contains a number of topics generated by the Editorial Board; additional topics can be proposed.  Those entries which have been published are in blue and are linked to the entry.  Assigned topics not yet completed are in black with the authors names.  Those without an assigned author are in black as title only. Members who wish to write on these topics should contact an Editor by email.
Instructions for authors can be reached here.

Integrative

 

  1. Measurement of pancreatic protein synthesis Maria Dolors Sans Gili, University of Michigan
  2. The isolated perfused pancreas
  3. Preparation of pancreatic fragments for studying the role of nerves and islets on pancreatic exocrine secretion Gino Saccone and Savio G. Barreto, Flinders Univ, Australia
  4. An isolated rat pancreas preparation for studying pancreatic spinal mechanosensitive and chemosensitive afferent activity Ann C. Schloithe, C.M. Woods and Gino Saccone, Flinders Univ, Australia
  5. Measurement of cholecystokinin Rodger Liddle, Duke University
  6. Quantitation of pancreatic blood flow by microspheres 
  7. Quantitation of autophagy in the pancreas at the tissue and cell levels Juan Iovanna and Maria Ines Vaccaro, Marseille France and Buenos Aires Argentina
  8. Isolation of Pancreatic RNA Robert C. De Lisle, University of Kansas
  9. Incorporation of polaxamer during tissue dissociation allows isolation of high quality RNA from FACS-sorted pancreatic cells Janivette Alsina, Steven Leach and Jennifer Bailey, Johns Hopkins University
  10. Lineage tracing in cells of the exocrine pancreas
  11. Zebrafish as a model organism for studying pancreatic development and function

Acinar Cell Studies

  1. Isolation of rodent pancreatic acinar cells and acini by collagenase digestion John Williams, University of Michigan
  2. Isolation of human pancreatic acinar cells from surgical tissue samples Matthew C. Cane1, Robert Sutton2 and David N. Criddle1, 1University of Liverpool and 2Royal Liverpool University Hospital
  3. Photoaffinity labeling of pancreatic receptors Maoqing Dong and Laurence J Miller, Mayo Scottsdale
  4. Measurement of intracellular calcium concentration in pancreatic acini Matthew J. Betzenhauser*, Jong Hak Wan, Hyungseo Park # and David Yule, *Columbia University Medical School, University of Rochester, #Konyang University
  5. Visualising the endoplasmic reticulum and its contacts with other organelles in live acinar cells   Hayley Dingsdale, Lee P Haynes, Alexei Tepikin and Gyorgy Lur, University of Liverpool
  6. Measuring Ca2+ dynamics in pancreatic acini using confocal microscopy Arbahim I. Orabi(1), Michael H. Nathanson(2) and Sohail Z. Husain(1), (1) University of Pittsburg, (2)Yale University
  7. Permeabilized acinar cells as a tool to study the effects of membrane impermeant agents on acinar exocytosis Diana DH Thomas and Guy E Groblewski, University of Wisconsin
  8. Knockdown of gene expression in isolated acini by siRNA and shRNA
  9. Expression of exogenous proteins in acini with adenoviral vectors
  10. Purification of pancreatic zymogen granules using percoll gradients Xuequn Chen and John A. Williams, University of Michigan
  11. Isolation, purification and protein content of pancreatic endoplasmic reticulum John A. Williams and Xuequn Chen, University of Michigan and Wayne State University
  12. Isolation of pancreatic mitochondria and measurement of their functional parameters Irina V. Odinokova (1,2), Natalia Shalbuyeva (1), Anna S. Gukovskaya(1) and Olga A. Mareninova (1), (1)UCLA and (2)Russian Academy of Sciences
  13. Chromatin Immunoprecipitation (ChIP) from pancreatic acinar cells and whole pancreatic tissue  Elena Fazio, Rashid Mehmood and Christopher Pin, University of Western Ontario
  14. Quantitation and visualization of protease activation in pancreatic acini Burkhard Kruger and Markus Lerch, University of Rostock, Germany, and Ernst-Moritz-Amdt University, Germany
  15. Quantitating activation of small G proteins in Ras and Rho families by pull down assays 
  16. Visualization of exocytosis in pancreatic acinar cells by fluorescence microscopy Yujin Jang and Peter Thorn, University of Queensland
  17. Immunofluorescent Surface Labeling of Externalized Pancreatic Zymogen Granules or Endolysosomal Vesicles Following Exocytosis Diana D.H Thomas and Guy E. Groblewski, University of Wisconsin

Duct Cell Studies

Consortium for Animal Models of Pancreatic Disease

Ed: Jami L. Saloman. University of Pittsburgh.
email: jls354@pitt.edu

The Pancreas Animal Models Cnsortium is a group of junior and senior scientist focused on fostering multidisciplinary collaboration as well as sharing knowledge and core resources. There is a large (and growing number of animal models of pancreas disease based on specific signaling pathways, genetic mutations, physical abnormalities, and environmental risk factors. The use of animal models can expand our understanding of the pathophysiology of multi-factorial pancreas diseases and aid in the development and validation of novel therapeutics. This collection of chapters on animal models of pancreas diseases has been developed for the purpose of providing up-to-date detailed information about the available models and how best to utilize them. Our broader objective is to develop consensus guidelines for required and optional endpoints in the analysis of pancreas animal models including pancreatitis and pancreatic cancer.

  1. Mouse Models of Trypsin-Dependent Pancreatitis. Miklós Sahin-Tóth, University of California Los Angeles
  2. Models of Pancreatitis Caused by Genetic Blockage of Autophagy/Lysosomal Pathway. Anna S. Gukovskaya,1,2 Olga A. Mareninova,1,2 Wen-Xing Ding,3 Aida Habtezion,4 and Ilya Gukovsky 1,2. (1) David Geffen School of Medicine, University of California at Los Angeles; (2) VA Greater Los Angeles Healthcare System, Los Angeles, CA; (3) Department of Pharmacology, Toxicology and Therapeutics, University of Kansas Medical Center, Kansas City, Kansas; (4) Division of Gastroenterology and Hepatology, Stanford University School of Medicine, Stanford, CA

Stellate Cell Studies

Digestive Enzymes and Digestion

Cell Lines and Primary Cell Cultures

  1. Culture of pancreatic acinar cells on a extracellular matrix
  2. Three dimensional culture of pancreatic acini
  3. Monolayer cultures of dog pancreatic duct cells as a model for pancreatic duct function Toan Nguyen, Thomas Wong, Christopher Savard and Sum P. Lee, University of Tennessee and Memphis VA Medical Center, University of Washington, Hong Kong University
  4. Culture of pancreatic AR42J cell for use as a model for acinar cell function Antonio Gonzalez, Patricia Santofimia-Castaño and Gines M. Salido, University of Extremadura, Spain
  5. Pancreatic cell lines derived from human pancreatic tumors

Pancreatic Disease Models

  1. Secretagogue (Caerulein) induced pancreatitis in rodents Julia Mayerle, Matthias Sendler, and Markus M. Lerch, Univ of Griefswald
  2. L-arginine-induced experimental acute pancreatitis Rajinder Dawra and Ashok Saluja, University of Minnesota
  3. Choline deficiency as a model for hemorrhagic pancreatitis
  4. Use of coxsackievirus B3 as model of acute pancreatitis Jonathan H. Hall and Dahn L. Clemens
  5. Retrograde infusion of bile acids into the pancreatic duct to induce pancreatitis in rats and mice George Perides, Gijs JD van Acker, Johanna Laukkarinen and Michael Steer, Tufts Medical Center
  6. Modeling alcoholic pancreatitis by ethanol feeding and lipopolysaccharide (LPS) challenge Alain Vonlaufen, University of Geneva
  7. Pancreatic duct ligation models of pancreatitis
  8. Quantitating inflammation in a mouse model of acute pancreatitis, Madhav Bhatia and  Hon Yen Lau, University of Otago, Christchurch
  9. Tumor-stromal interactions assessed by co-injection of pancreatic stellate and cancer cells Rosa Hwang, M.D. Anderson

Human Studies