Acinar Cell Studies

  1. Isolation of rodent pancreatic acinar cells and acini by collagenase digestion John Williams, University of Michigan
  2. Isolation of human pancreatic acinar cells from surgical tissue samples Matthew C. Cane1, Robert Sutton2 and David N. Criddle1, 1University of Liverpool and 2Royal Liverpool University Hospital
  3. Photoaffinity labeling of pancreatic receptors Maoqing Dong and Laurence J Miller, Mayo Scottsdale
  4. Measurement of intracellular calcium concentration in pancreatic acini Matthew J. Betzenhauser*, Jong Hak Wan, Hyungseo Park # and David Yule, *Columbia University Medical School, University of Rochester, #Konyang University
  5. Visualising the endoplasmic reticulum and its contacts with other organelles in live acinar cells   Hayley Dingsdale, Lee P Haynes, Alexei Tepikin and Gyorgy Lur, University of Liverpool
  6. Measuring Ca2+ dynamics in pancreatic acini using confocal microscopy Arbahim I. Orabi(1), Michael H. Nathanson(2) and Sohail Z. Husain(1), (1) University of Pittsburg, (2)Yale University
  7. Permeabilized acinar cells as a tool to study the effects of membrane impermeant agents on acinar exocytosis Diana DH Thomas and Guy E Groblewski, University of Wisconsin
  8. Knockdown of gene expression in isolated acini by siRNA and shRNA
  9. Expression of exogenous proteins in acini with adenoviral vectors
  10. Purification of pancreatic zymogen granules using percoll gradients Xuequn Chen and John A. Williams, University of Michigan
  11. Isolation, purification and protein content of pancreatic endoplasmic reticulum John A. Williams and Xuequn Chen, University of Michigan and Wayne State University
  12. Isolation of pancreatic mitochondria and measurement of their functional parameters Irina V. Odinokova (1,2), Natalia Shalbuyeva (1), Anna S. Gukovskaya(1) and Olga A. Mareninova (1), (1)UCLA and (2)Russian Academy of Sciences
  13. Chromatin Immunoprecipitation (ChIP) from pancreatic acinar cells and whole pancreatic tissue  Elena Fazio, Rashid Mehmood and Christopher Pin, University of Western Ontario
  14. Quantitation and visualization of protease activation in pancreatic acini Burkhard Kruger and Markus Lerch, University of Rostock, Germany, and Ernst-Moritz-Amdt University, Germany
  15. Quantitating activation of small G proteins in Ras and Rho families by pull down assays 
  16. Visualization of exocytosis in pancreatic acinar cells by fluorescence microscopy Yujin Jang and Peter Thorn, University of Queensland
  17. Immunofluorescent Surface Labeling of Externalized Pancreatic Zymogen Granules or Endolysosomal Vesicles Following Exocytosis Diana D.H Thomas and Guy E. Groblewski, University of Wisconsin