Description (including epitope tag if present):
N-terminal HA tagged human RGS2 from the University of Missouri Rollo was subcloned into the Adtrack-CMV vector and recombined with pAdEasy-1 in BJ5183 strain of E. coli.
Constructed by Patrick J Casey, Duke University. Available from Dr. Williams with permission from Dr. Casey
Has been used to inhibit Gq signaling in prostate cancer cells and mouse pancreatic acinar cells. Does not affect GaS or Ga13
Publications describing the vector and usage:
- Kelly, P, LN Stemmle, JF Madden, TA Fields, Y Daaka, and PJ Casey. A role for the G12 family of heterotrimeric G proteins in prostate cancer invasion. J Biol Chem 281:26483-26490, 2006
- Sabbatini, ME, Y Bi, B Ji, SA Ernst, and JA Williams. CCK activates RhoA and Rac1 differentially through Ga13 and Gaq in mouse pancreatic acini. Am J Physiol Cell Physiol. 298:C592-C601, 2010.