Description (including epitope tag if present):
Myc-p115RGS from Dr. Tohru Kosaza of Northwestern University which contains the RGS-like domain from p115RhoGEF was used to prepare a adenoviral vector using the AdtrackCMV vector and the AdEasy system.
Pactrick J Casey of Duke University. Vector can be obtained from Dr. Williams with permission from Dr Casey.
Binds to Ga12/13 and inhibits their signaling in prostate cancer and mouse pancreatic acinar cells.
Publications describing the vector and usage:
- Kelly, P, LN Stemmle, JF Madden, TA Fields, Y Daaka, and PJ Casey. A role for the G12 family of heterotrimeric G proteins in prostate cancer invasion. J Biol Chem 281:26483-26490, 2006
- Sabbatini, ME, Y Bi, B Ji, SA Ernst, and JA Williams. CCK activates RhoA and Rac1 differentially through Ga13 and Gaq in mouse pancreatic acini. Am J Physiol Cell Physiol. 298:C592-C601, 2010.