Full name (if applicable):
Galpha 13 (Q226L)
Description (including epitope tag if present):
The mouse sequence for Gα13 with a Q226L mutation was obtained from Diane Barber (U C San Francisco) and used to prepare adenovirus using the pAdTrack system by Yan Bi in the Williams lab.
The viral vector has been used to overexpress active G13 in mouse pancreatic cells. Overexpressed protein can be demonstrated by Western blotting using a rabbit Ab to G13 from Santa Cruz after 12 to 14 hours. This was accompanied by activation of RhoA, disruption of the filamentous actin cytoskeleton and formation of basolateral blebs.
Publications describing the vector and usage:
Sabbatini ME, Bi y, Ji, B, Ernst, SA, and Williams, JA. CCK activates Rho A and Rac1 differentially through Gα13 and Gαq in mouse pancreatic acini. Am J Physiol Cell Physiol 298:C592-C601, 2010.