Pancreapedia: Exocrine Pancreas Knowledge Base, DOI: 10.3998/panc.2015.28
Excessive alcohol consumption is a major contributor to diverse pathologies with an estimated 4 in 100 deaths worldwide caused by alcohol according to the World Health Organization (65). The close association between alcohol consumption and acute pancreatitis (AP) has been recognized for a long time, with Friedrich first describing the Drunkard's Pancreas in 1878, although elevated intake of alcohol had been linked to pancreatic disease a century earlier (4). More recently, a Danish population-based cohort study has shown an increased risk of AP in individuals who consumed in excess of 14 drinks per week, irrespective of the type of beverage or frequency of intake (Kristiansen et al., 2008). A subsequent meta-analysis indicated that an elevated risk of AP exists in those imbibing greater than 4 drinks per day (26). Despite the recognized risk of AP increasing with alcohol intake its basis remains incompletely understood and no specific therapy exists (52). Intriguingly, some individuals appear more susceptible to developing AP linked to excess alcohol consumption than others, with less than 10% of heavy drinkers developing clinical disease. However, this phenomenon has no clear explanation and clearly is an important area for investigation. Progress in elucidating the pathophysiology of alcoholic AP has been complicated by the fact that alcohol alone does not induce AP reliably in experimental animal models, with additional factors required to model alcohol-induced pancreatic inflammation and damage, including caerulein, lipopolysaccharide and ductal obstruction that may not accurately reflect the clinical situation (34). Direct sensitizing actions of ethanol are thought to contribute to damaging effects (50), including activation of NFκB in pancreatic acinar cells via the ε isoform of protein kinase C (58) and activation of cholinergic pathways (36). Recent work has focused on the way in which alcohol metabolism may be involved in mediating pancreatic toxicity.
2. Ethanol Metabolism
Ethanol is metabolized in the pancreas by both oxidative and non-oxidative routes (5, 16). Current evidence indicates that both pathways are likely to contribute to the detrimental effects of alcohol on the exocrine pancreas, via distinct mechanisms that ultimately compromise mitochondrial function (7, 9, 44, 60). Oxidative metabolism (OME) proceeds through several nicotinamide adenine dinucleotide (NAD+) consuming steps performed by alcohol and aldehyde dehydrogenases (ADH and ALDH) that generate acetaldehyde and acetate, respectively. Recent findings have suggested that ethanol induces mitochondrial dysfunction via a reduction of the ratio of oxidized to reduced nicotinamide adenine dinucleotide, a mechanism distinct from the effects of cholecystokinin hyperstimulation which are mediated by increasing cytosolic calcium ([Ca2+]c) (54, 60).
In contrast to oxidative metabolism of ethanol (OME), non-oxidative metabolism of ethanol (NOME) promotes esterification of fatty acids to yield highly lipophilic fatty acid ethyl esters (FAEEs) via FAEE synthases including carboxylester lipase (CEL). FAEE synthase activity occurs in the human pancreas at rates of up to 54 nmol/min/g tissue, generating high localized levels of FAEEs (12). An autopsy study showed that individuals who died of acute alcohol intoxication had preferentially elevated FAEEs in the pancreas, in contrast to other organs commonly damaged by alcohol such as the heart and lungs (32), suggesting importance of NOME to pancreatic damage. In vivo studies in rats subsequently confirmed that saturated FAEEs induced pancreatic damage indicative of AP (63). Furthermore, administration of ethanol, under conditions of OME inhibition, generated plasma and tissue FAEEs and development of AP (64). Early (<15 mins.) redistribution of CEL into the cytosol from a predominantly apical, granular localization within the pancreatic acinar cell occurred following in vivo administration of fat and alcohol to mice in a new in vivo model of alcoholic AP (FAEE-AP) (24). Furthermore, inhibition of CEL blocked FAEE generation and ameliorated detrimental effects of fat and alcohol (24). In AP patients, elevated CEL is detectable in necrotic pancreatic lobules and in areas of fat necrosis (1), consistent with localized generation of toxic FAEEs in areas of damage. In pancreatic acinar cells FAEEs released calcium from the endoplasmic reticulum (ER) via stimulation of inositol trisphosphate (IP3) receptors, causing a depletion of internal calcium stores that led to store-operated calcium entry (SOCE), promoting toxic, sustained elevations of cytosolic calcium ([Ca2+]C) that lead to necrotic cell death (7, 9, 10). Furthermore, FAEEs underwent hydrolysis to fatty acids in the mitochondria causing a localized elevation that compromised mitochondrial function (9, 25, 31). Pharmacological inhibition of hydrolase enzymes significantly reduced necrosis induced by a fat and alcohol combination, highlighting the importance of fatty acid release in the mitochondria to cellular damage (10). Diverse actions of FAEEs have been reported in pancreas including increased fragility of lysosomes (19) and inhibition of serine proteases (37) that may predispose to fibrogenesis and impaired pancreas recovery after organ damage in chronic injury (38).
Recent progress in understanding the basis of alcohol-induced damage has highlighted the importance of organellar dysfunction within the pancreatic acinar cell as central for initiation of AP. In particular, the involvement of mitochondria and the endoplasmic reticulum, two organelles that are intimately linked spatially and functionally to modulate cellular calcium homeostasis, energy production, and lipid and protein synthesis (Figure 1) (11, 27, 55).
3. Mitochondrial Dysfunction in Alcoholic Acute Pancreatitis
Mitochondria perform a variety of tasks in the pancreatic acinar cell, the most important being provision of energy for cellular processes including secretion of inactive digestive enzyme precursors. In order to do this effectively mitochondria respond to oscillatory rises of [Ca2+]C, induced by hormonal (cholecystokinin) and neuronal (acetylcholine) stimulation (6, 45, 62), by generating NADH, via stimulation of Ca2+-dependent dehydrogenases of the Krebs Cycle, that feeds into the electron transport chain to promote ATP production. Additionally, mitochondria are thought to constitute a protective perigranular buffer barrier that impedes movement of excessive Ca2+ released from the apical pole to the basolateral region in which the nucleus resides (53). However, when sustained rises of [Ca2+]C occur in pancreatic acinar cells in response to aberrant Ca2+ signals induced by diverse AP precipitants, including CCK hyperstimulation, bile salts and ethanol metabolites, mitochondrial dysfunction ensues that leads to rundown of ATP production and induction of cellular necrosis (8, 10, 18, 43).
Recent evidence has shown that the trigger for mitochondrial dysfunction in AP is the opening of the mitochondrial permeability transition pore (MPTP) (44, 60), which permeabilizes the inner mitochondrial membrane allowing free movement of substances up to 1.5 kDa in and out of the organelle. MPTP formation thus leads to collapse of membrane potential, dissipating the proton gradient necessary for production of ATP. Although the exact composition of the pore remains controversial, recent evidence has indicated that it may be a dimer of the F0/F1-ATP synthase (2, 14). In response to AP precipitants in both human and murine pancreatic acinar cells, calcium-dependent MPTP formation occurred as a consequence of IP3 and ryanodine receptor-mediated intracellular calcium release and subsequent SOCE; diminished ATP production led to impaired calcium clearance, defective autophagy, zymogen activation, cytokine production, phosphoglycerate mutase 5 activation and necrosis (44). The crucial role played by compromised intracellular ATP levels as a result of mitochondrial dysfunction has been shown by studies in which detrimental effects of AP toxins, including non-oxidative ethanol metabolites, were preventable by intracellular ATP supplementation in isolated pancreatic acinar cells, allowing energy-dependent calcium extrusion pumps to reduce [Ca2+]C and maintain homeostasis (3, 9, 44). The mitochondrial matrix protein peptidyl-prolyl cis-trans isomerase cyclophilin D (CypD) plays a pivotal role in modulating the MPTP; all biochemical, immunological and histopathological responses of AP in four experimental models, including alcoholic (FAEE-AP), were reduced or abolished by genetic deletion or pharmacological modulation of this protein (44), suggesting the potential of CypD inhibitors for translational therapy.
4. Endoplasmic Reticulum Responses with Alcohol
The endoplasmic reticulum (ER) of the pancreatic acinar cell plays a predominant role in the function of the cell as protein synthesis and transport are highly developed in this cell. It is not surprising then that the ER responds to alcohol. A previous study has shown that exposure of pancreatic acinar cells to ethanol induced a slow, gradual release of calcium from the ER (10). The ER not only translates mRNA into new proteins synthesized in its lumen but it performs several post-translational modifications including disulfide bond formation facilitated by chaperone-mediated protein folding and other post-translational modifications such as glycosylation. Correctly folded and otherwise modified proteins are directed to specific cellular organelles. As an example, the digestive enzyme proteins are segregated into the secretory pathway ending up in zymogen granules which undergo exocytosis and secretion with neurohormonal stimulation. As another example, acid hydrolases are glycosylated with mannose-6-phosphate which is necessary for their transport to the lysosome.
In general protein folding is accomplished in the ER by molecular chaperones and folding enzymes that include disulfide isomerases and oxidoreductases. In addition, there is a quality control mechanism that degrades improperly processed proteins by proteosomal degradation. This process is called ER-associated degradation (ERAD). Autophagy also participates in degradation of dysfunctional ER and damaged or misfolded proteins to prevent cellular toxicity that these proteins may cause. (13, 29)
In order to adjust to changing demands encountered by the ER protein synthesis and processing machinery including ethanol and its metabolism, eukaryotic cells have developed a complex signaling system referred to as the Unfolded Protein Response (UPR). Activation of the UPR occurs with accumulated unfolded or misfolded proteins in the ER lumen, a phenomenon termed “ER stress” (56). ER stress has several sources, including a physiologic increase in the demand for protein folding; decreased chaperone function; accumulation of permanently misfolded proteins due to mutation; decreases in cellular ATP levels or a fall in calcium in the ER ([Ca2+]ER); and perturbed ER redox status that occurs with alcohol metabolism (39, 48). Interestingly, the non-oxidative ethanol metabolite palmitoleic acid ethyl ester and palmitoleic acid, which is released by hydrolysis of its parent FAEE (24), caused complete depletion of [Ca2+]ER, with concomitant falls of NADH and depletion of cellular ATP (Figure 1) (9, 10). Also, the folding process itself generates reactive oxygen species which, in turn, can cause aberrant disulfide bond formation (i.e. misfolding). Thus, in the case of a continuous misfolding stress as occurs with mutation or possibly with ethanol metabolism there will be a greater ER stress than would occur during a transient increase in unfolded proteins as a consequence of the need to replenish zymogen stores.
The UPR has three major response systems to ER stress. These are a global reduction in mRNA translation which attenuates the demand for protein processing; an increase in expression of chaperones and foldases as well as increased phospholipid synthesis to expand the functional ER network; and activation of the ERAD and autophagic systems to eliminate misfolded and aberrant proteins (28, 29, 42, 56, 57). These responses are accomplished by identified sensing and signaling systems (56, 57). They include Inositol-requiring protein-1α (IRE1α), activating transcription factor-6 (ATF6), and RNA-activated protein kinase (PKR)-like ER kinase (PERK).
Regarding alcohol-induced ER stress, we found a key role for IRE1α in preventing damage to the exocrine pancreas (39, 47). Upon its activation, an endonuclease activity within IRE1α splices X-box binding protein-1 (XBP1) mRNA resulting in a shorter mRNA (spliced XBP1, sXBP1 mRNA) that encodes the active transcription factor sXBP1. sXBP1 regulates a broad spectrum of genes involved in protein folding, including chaperones, disulfide isomerases and oxidoreductases family as well as genes for protein degradation (ERAD), lipid biosynthesis for ER/Golgi biogenesis, vesicular trafficking and redox metabolism (28, 33). In the exocrine pancreas, sXBP1 is especially necessary for acinar cell homeostasis and function (33). The critical importance of sXBP1 for the function of the pancreatic acinar cell is supported by studies using Xbp1+/- mice (39, 40) and acinar cell specific Xbp1 null mice (23, 33). XBP1 deficiency results in defective stimulated secretory response, extensive acinar cell loss and inflammation as well as severe pathology in the remaining acinar cells, as evidenced by reduced levels of ER chaperones, a poorly developed ER network and secretory system, marked reduction in zymogen granules and digestive enzymes, and accumulation of autophagic vacuoles.(23, 40)
Ethanol feeding in rodents induces structural changes in the acinar cell consistent with ER stress such as ER dilation, mitochondrial swelling and some disorganization of cellular organelles.(17, 30, 39) However, chronic ethanol-fed animals, as humans, do not develop pancreatitis unless challenged with other toxic factors.(49, 51, 61) We found that pancreatic mRNA and protein levels of sXBP1 were significantly increased in mouse and rats fed ethanol-containing diets.(39). In order to determine whether the upregulation of sXBP1 by alcohol feeding is necessary to maintain homeostasis and prevent pancreatitis, we used Xbp1 heterozygous mice (Xbp+/-). Compared to ethanol-fed wild-type mice (Xbp1+/+), histological analysis of pancreatic tissue in ethanol-fed Xbp1+/- mice revealed morphologic features of severe ER stress such as disorganized and dilated ER, accumulation of dense material within the ER, and a reduced number of mature zymogen granules. These features were accompanied by accumulation of autophagic vacuoles, and activation of apoptotic signals including upregulation of CHOP (see below) within patchy areas of inflammatory pancreatitis.(39, 40) Moreover, recent studies indicate that cerulein-induced AP is more severe in XBP1 deficient mice compare to controls (unpublished observations). From these studies we concluded that alcohol feeding activates an adaptive and protective UPR through increased expression of sXBP1 involving activation of the endonuclease activity of IRE1α. Further, these actions of the UPR are necessary to prevent cellular toxicities of ethanol.
Whereas IRE1α/XBP1 signaling primarily mediates adaptive responses to protect ER function, this protective signal can be prematurely attenuated during severe or prolong ER stress resulting in upregulation of proapoptotic cell death mediated through the transcription factor, C/EBP homologous protein (CHOP)(35) as discussed in more detail below. Also, genetic inhibition of Xbp1 is unequivocally associated with potent upregulation of CHOP and cell death (33, 39). On the other hand, forced and sustained IRE1α/XBP1 activity enhances cell survival in conditions of severe stress (35), further supporting a protective role for sXBP1 signaling.
The PERK UPR branch has a dual role. Firstly, when activated it rapidly adjusts the cell to ER stress by mediating a general attenuation of protein synthesis (20, 21, 56, 59). On the hand, sustained activation of PERK leads to upregulation of the transcription factor, Activating transcription factor 4 (ATF4) that targets genes involved in antioxidant activities including glutathione synthesis (22) and CHOP that promotes ER stress-related cell death responses (46). CHOP also promotes inflammation by regulating cytokine production and promoting the survival of inflammatory cells (15, 41). In summary, although the PERK activation can play a transient protective role, unresolved ER stresses leads to upregulation of CHOP and promotion of inflammation and pancreatitis.
This chapter reviews two bodies of work related to alcohol effects on the exocrine pancreas. One addresses mitochondrial functional changes and the other endoplasmic reticulum responses, phenomena that may be interrelated in acute pancreatitis (Figure 1). Alcohol-induced disorders of both organelles make the pancreas susceptible to alcohol-induced injury and recent advances suggest the potential for translational therapy. Interestingly, there are some protective responses coming from the endoplasmic reticulum UPR that may be a reason why only a minority of drinkers develop pancreatitis.
Acknowledgements: Support was provided by MRC grant KO12967; NIHR Biomedical Research Unit funding scheme; NIH grants P01DK098108, P50AA011999 and R01AA019954; and the Department of Veterans Affairs.
- Aho HJ,Sternby B,Kallajoki M and Nevalainen TJ. Carboxyl ester lipase in human tissues and in acute pancreatitis. Int J Pancreatol 5(2): 123-134,1989. PMID: 2689525.
- Bonora M,Bononi A,De Marchi E,Giorgi C,Lebiedzinska M,Marchi S, et al. Role of the c subunit of the FO ATP synthase in mitochondrial permeability transition. Cell Cycle 12(4): 674-683,2013. PMID: 23343770.
- Booth DM,Murphy JA,Mukherjee R,Awais M,Neoptolemos JP,Gerasimenko OV, et al. Reactive oxygen species induced by bile acid induce apoptosis and protect against necrosis in pancreatic acinar cells. Gastroenterology 140(7): 2116-2125,2011. PMID: 21354148.
- Cawley TA. A singular case of diabetes, consisting entirely in the quantity of urine with an enquiry into the different theories of that disease. Lond. Med. J. 9: 286,1788.
- Criddle DN. The role of fat and alcohol in acute pancreatitis: A dangerous liaison. Pancreatology,2015. PMID: 25845855.
- Criddle DN,Booth DM,Mukherjee R,McLaughlin E,Green GM,Sutton R, et al. Cholecystokinin-58 and cholecystokinin-8 exhibit similar actions on calcium signaling, zymogen secretion, and cell fate in murine pancreatic acinar cells. Am J Physiol Gastrointest Liver Physiol 297(6): G1085-1092,2009. PMID: 19815626.
- Criddle DN,Gerasimenko JV,Baumgartner HK,Jaffar M,Voronina S,Sutton R, et al. Calcium signalling and pancreatic cell death: apoptosis or necrosis? Cell Death Differ 14(7): 1285-1294,2007. PMID: 17431416.
- Criddle DN,Murphy J,Fistetto G,Barrow S,Tepikin AV,Neoptolemos JP, et al. Fatty Acid Ethyl Esters Cause Pancreatic Calcium Toxicity via Inositol Trisphosphate Receptors and Loss of ATP Synthesis. Gastroenterology 130(3): 781-793,2006. PMID: 16530519.
- Criddle DN,Raraty MG,Neoptolemos JP,Tepikin AV,Petersen OH and Sutton R. Ethanol toxicity in pancreatic acinar cells: mediation by nonoxidative fatty acid metabolites. Proc Natl Acad Sci USA 101(29): 10738-10743,2004. PMID: 15247419.
- Csordas G,Thomas AP and Hajnoczky G. Quasi-synaptic calcium signal transmission between endoplasmic reticulum and mitochondria. EMBO J 18(1): 96-108,1999. PMID: 9878054.
- Diczfalusy MA,Bjorkhem I,Einarsson C,Hillebrant CG and Alexson SE. Characterization of enzymes involved in formation of ethyl esters of long-chain fatty acids in humans. J Lipid Res 42(7): 1025-1032,2001. PMID: 11441128.
- Direnzo D,Hess DA,Damsz B,Hallet JE,Marshall B,Goswami C, et al. Induced MIST1 Expression Promotes Remodeling Of Mouse Pancreatic Acinar Cells. Gastroenterology,2012. PMID: 22510200.
- Giorgio V,von Stockum S,Antoniel M,Fabbro A,Fogolari F,Forte M, et al. Dimers of mitochondrial ATP synthase form the permeability transition pore. Proc Natl Acad Sci USA 110(15): 5887-5892,2013. PMID: 23530243.
- Goodall JC,Wu C,Zhang Y,McNeill L,Ellis L,Saudek V, et al. Endoplasmic reticulum stress-induced transcription factor, CHOP, is crucial for dendritic cell IL-23 expression. Proc Natl Acad Sci USA 107(41): 17698-17703,2010. PMID: 20876114.
- Gukovskaya AS,Mouria M,Gukovsky I,Reyes CN,Kasho VN,Faller LD, et al. Ethanol metabolism and transcription factor activation in pancreatic acinar cells in rats. Gastroenterology 122(1): 106-118,2002. PMID: 11781286.
- Gukovsky I,Lugea A,Shahsahebi M,Cheng JH,Hong PP,Jung YJ, et al. A rat model reproducing key pathological responses of alcoholic chronic pancreatitis. Am J Physiol Gastrointest Liver Physiol 294(1): G68-79,2008. PMID: 17884979.
- Gukovsky I,Pandol SJ and Gukovskaya AS. Organellar dysfunction in the pathogenesis of pancreatitis. Antioxid Redox Signal 15(10): 2699-2710,2011. PMID: 21834686.
- Haber PS,Wilson JS,Apte MV and Pirola RC. Fatty acid ethyl esters increase rat pancreatic lysosomal fragility. J Lab Clin Med 121(6): 759-764,1993. PMID: 8505587.
- Han J,Back SH,Hur J,Lin YH,Gildersleeve R,Shan J, et al. ER-stress-induced transcriptional regulation increases protein synthesis leading to cell death. Nat Cell Biol 15(5): 481-490,2013. PMID: 23624402.
- Harding HP,Novoa I,Zhang Y,Zeng H,Wek R,Schapira M, et al. Regulated translation initiation controls stress-induced gene expression in mammalian cells. Mol Cell 6(5): 1099-1108,2000. PMID: 11106749.
- Harding HP,Zhang Y,Zeng H,Novoa I,Lu PD,Calfon M, et al. An integrated stress response regulates amino acid metabolism and resistance to oxidative stress. Mol Cell 11(3): 619-633,2003. PMID: 12667446.
- Hess DA,Humphrey SE,Ishibashi J,Damsz B,Lee AH,Glimcher LH, et al. Extensive pancreas regeneration following acinar-specific disruption of Xbp1 in mice. Gastroenterology 141(4): 1463-1472,2011. PMID: 21704586.
- Huang W,Booth DM,Cane MC,Chvanov M,Javed MA,Elliott VL, et al. Fatty acid ethyl ester synthase inhibition ameliorates ethanol-induced Ca2+-dependent mitochondrial dysfunction and acute pancreatitis. Gut 63(8): 1313-1324,2014. PMID: 24162590.
- Huang W,Cash N,Wen L,Szatmary P,Mukherjee R,Armstrong J, et al. Effects of the mitochondria-targeted antioxidant mitoquinone in murine acute pancreatitis. Mediators Inflamm 2015: 901780,2015. PMID: 25878403.
- Irving HM,Samokhvalov AV and Rehm J. Alcohol as a risk factor for pancreatitis. A systematic review and meta-analysis. JOP 10(4): 387-392,2009. PMID: 19581740.
- Johnson PR,Dolman NJ,Pope M,Vaillant C,Petersen OH,Tepikin AV, et al. Non-uniform distribution of mitochondria in pancreatic acinar cells. Cell Tissue Res 313(1): 37-45,2003. PMID: 12838407.
- Kaser A,Lee AH,Franke A,Glickman JN,Zeissig S,Tilg H, et al. XBP1 links ER stress to intestinal inflammation and confers genetic risk for human inflammatory bowel disease. Cell 134(5): 743-756,2008. PMID: 18775308.
- Kim I,Xu W and Reed JC. Cell death and endoplasmic reticulum stress: disease relevance and therapeutic opportunities. Nat Rev Drug Discov 7(12): 1013-1030,2008. PMID: 19043451.
- Lam PP,Cosen Binker LI,Lugea A,Pandol SJ and Gaisano HY. Alcohol redirects CCK-mediated apical exocytosis to the acinar basolateral membrane in alcoholic pancreatitis. Traffic 8(5): 605-617,2007. PMID: 17451559.
- Lange LG and Sobel BE. Mitochondrial dysfunction induced by fatty acid ethyl esters, myocardial metabolites of ethanol. J Clin Invest 72(2): 724-731,1983. PMID: 6308061.
- Laposata EA and Lange LG. Presence of nonoxidative ethanol metabolism in human organs commonly damaged by ethanol abuse. Science 231(4737): 497-499,1986. PMID: 3941913.
- Lee AH,Chu GC,Iwakoshi NN and Glimcher LH. XBP-1 is required for biogenesis of cellular secretory machinery of exocrine glands. EMBO J 24(24): 4368-4380,2005. PMID: 16362047.
- Lerch MM and Gorelick FS. Models of acute and chronic pancreatitis. Gastroenterology 144(6): 1180-1193,2013. PMID: 23622127.
- Lin JH,Li H,Yasumura D,Cohen HR,Zhang C,Panning B, et al. IRE1 signaling affects cell fate during the unfolded protein response. Science 318(5852): 944-949,2007. PMID: 17991856.
- Lugea A,Gong J,Nguyen J,Nieto J,French SW and Pandol SJ. Cholinergic mediation of alcohol-induced experimental pancreatitis. Alcohol Clin Exp Res 34(10): 1768-1781,2010. PMID: 20626730.
- Lugea A,Gukovsky I,Gukovskaya AS and Pandol SJ. Nonoxidative ethanol metabolites alter extracellular matrix protein content in rat pancreas. Gastroenterology 125(6): 1845-1859,2003. PMID: 14724836.
- Lugea A,Nan L,French SW,Bezerra JA,Gukovskaya AS and Pandol SJ. Pancreas recovery following cerulein-induced pancreatitis is impaired in plasminogen-deficient mice. Gastroenterology 131(3): 885-899,2006. PMID: 16952557.
- Lugea A,Tischler D,Nguyen J,Gong J,Gukovsky I,French SW, et al. Adaptive unfolded protein response attenuates alcohol-induced pancreatic damage. Gastroenterology 140(3): 987-997,2011. PMID: 21111739.
- Lugea A,Waldron RT,French SW and Pandol SJ. Drinking and driving pancreatitis: links between endoplasmic reticulum stress and autophagy. Autophagy 7(7): 783-785,2011. PMID: 21460613.
- Malhi H,Kropp EM,Clavo VF,Kobrossi CR,Han J,Mauer AS, et al. C/EBP Homologous Protein-induced Macrophage Apoptosis Protects Mice from Steatohepatitis. J Biol Chem 288(26): 18624-18642,2013. PMID: 23720735.
- Marciniak SJ,Garcia-Bonilla L,Hu J,Harding HP and Ron D. Activation-dependent substrate recruitment by the eukaryotic translation initiation factor 2 kinase PERK. J Cell Biol 172(2): 201-209,2006. PMID: 16418533.
- Mukherjee R,Criddle DN,Gukvoskaya A,Pandol S,Petersen OH and Sutton R. Mitochondrial injury in pancreatitis. Cell Calcium,2008. PMID: 18207570.
- Mukherjee R,Mareninova OA,Odinokova IV,Huang W,Murphy J,Chvanov M, et al. Mechanism of mitochondrial permeability transition pore induction and damage in the pancreas: inhibition prevents acute pancreatitis by protecting production of ATP. Gut,2015. PMID: 26071131.
- Murphy JA,Criddle DN,Sherwood M,Chvanov M,Mukherjee R,McLaughlin E, et al. Direct activation of cytosolic Ca2+ signaling and enzyme secretion by cholecystokinin in human pancreatic acinar cells. Gastroenterology 135(2): 632-641,2008. PMID: 18555802.
- Oyadomari S and Mori M. Roles of CHOP/GADD153 in endoplasmic reticulum stress. Cell Death Differ 11(4): 381-389,2004. PMID: 14685163.
- Pandol SJ,Gorelick FS,Gerloff A and Lugea A. Alcohol abuse, endoplasmic reticulum stress and pancreatitis. Dig Dis 28(6): 776-782,2010. PMID: 21525762.
- Pandol SJ,Gorelick FS and Lugea A. Environmental and genetic stressors and the unfolded protein response in exocrine pancreatic function - a hypothesis. Front Physiol 2: 8,2011. PMID: 21483727.
- Pandol SJ,Gukovsky I,Satoh A,Lugea A and Gukovskaya AS. Animal and in vitro models of alcoholic pancreatitis: role of cholecystokinin. Pancreas 27(4): 297-300,2003. PMID: 14576490.
- Pandol SJ,Lugea A,Mareninova OA,Smoot D,Gorelick FS,Gukovskaya AS, et al. Investigating the pathobiology of alcoholic pancreatitis. Alcohol Clin Exp Res 35(5): 830-837,2011. PMID: 21284675.
- Pandol SJ,Periskic S,Gukovsky I,Zaninovic V,Jung Y,Zong Y, et al. Ethanol diet increases the sensitivity of rats to pancreatitis induced by cholecystokinin octapeptide. Gastroenterology 117(3): 706-716,1999. PMID: 10464148.
- Pandol SJ,Saluja AK,Imrie CW and Banks PA. Acute pancreatitis: bench to the bedside. Gastroenterology 133(3): 1056-1056,2007. PMID: 17383433.
- Petersen OH and Tepikin AV. Polarized Calcium Signaling in Exocrine Gland Cells. Annual Review of Physiology 70(1): 273-299,2008. PMID: 17850212.
- Raraty M,Ward J,Erdemli G,Vaillant C,Neoptolemos JP,Sutton R, et al. Calcium-dependent enzyme activation and vacuole formation in the apical granular region of pancreatic acinar cells. Proc Natl Acad Sci USA 97(24): 13126-13131,2000. PMID: 11087863.
- Rizzuto R,Pinton P,Carrington W,Fay FS,Fogarty KE,Lifshitz LM, et al. Close contacts with the endoplasmic reticulum as determinants of mitochondrial Ca2+ responses. Science 280(5370): 1763-1766,1998. PMID: 9624056.
- Ron D and Walter P. Signal integration in the endoplasmic reticulum unfolded protein response. Nat Rev Mol Cell Biol 8(7): 519-529,2007. PMID: 17565364.
- Rutkowski DT and Kaufman RJ. That which does not kill me makes me stronger: adapting to chronic ER stress. Trends Biochem Sci 32(10): 469-476,2007. PMID: 17920280.
- Satoh A,Gukovskaya AS,Reeve JR, Jr.,Shimosegawa T and Pandol SJ. Ethanol sensitizes NF-kappaB activation in pancreatic acinar cells through effects on protein kinase C-epsilon. Am J Physiol Gastrointest Liver Physiol 291(3): G432-G438,2006. PMID: 16574982.
- Scheuner D,Song B,McEwen E,Liu C,Laybutt R,Gillespie P, et al. Translational control is required for the unfolded protein response and in vivo glucose homeostasis. Mol Cell 7(6): 1165-1176,2001. PMID: 11430820.
- Shalbueva N,Mareninova OA,Gerloff A,Yuan J,Waldron RT,Pandol SJ, et al. Effects of oxidative alcohol metabolism on the mitochondrial permeability transition pore and necrosis in a mouse model of alcoholic pancreatitis. Gastroenterology 144(2): 437-446 e436,2013. PMID: 23103769.
- Vonlaufen A,Phillips PA,Xu Z,Zhang X,Yang L,Pirola RC, et al. Withdrawal of alcohol promotes regression while continued alcohol intake promotes persistence of LPS-induced pancreatic injury in alcohol-fed rats. Gut 60(2): 238-246,2011. PMID: 20870739.
- Voronina S,Sukhomlin T,Johnson PR,Erdemli G,Petersen OH and Tepikin A. Correlation of NADH and Ca2+ signals in mouse pancreatic acinar cells. J Physiol 539(Pt 1): 41-52,2002. PMID.
- Werner J,Laposata M,Fernandez-del Castillo C,Saghir M,Iozzo RV,Lewandrowski KB, et al. Pancreatic injury in rats induced by fatty acid ethyl ester, a nonoxidative metabolite of alcohol. Gastroenterology 113(1): 286-294,1997. PMID: 11850500.
- Werner J,Saghir M,Warshaw AL,Lewandrowski KB,Laposata M,Iozzo RV, et al. Alcoholic pancreatitis in rats: injury from nonoxidative metabolites of ethanol. Am J Physiol Gastrointest Liver Physiol 283(1): G65-G73,2002. PMID: 12065293.
- Zarocostas J. Four in 100 deaths worldwide are caused by alcohol, says WHO. Br Med J 342:d1032. 2011. PMID: 21321011.