Description (including epitope tag if present):
NFAT-luciferase reporter (9 multimerized NFAT binding sites upstream of a minimal TATA-containing promoter fused to luciferase) was cloned into the multiple cloning site of pAC-CMV-pLpA construct. The resulting plasmid was cotransfected into HEK293 cells with plasmid pJM17 which contains the Ad5 genome.
Source:
How used:
Optimized and performed in isolated, primary mouse acinar cells as well as secondary cell lines including
acinar-derived AR42J, 266-6 and non-acinar HEK293 and NIH 3T3. Please refer to methods section in pancreapedia "Quantitative assays of transcriptional activity: NFAT-Luciferase reporter".
Publications describing the vector and usage:
- Cholecystokinin activates pancreatic calcineurin-NFAT signaling in vitro and in vivo. Gurda GT, Guo L, Lee SH, Molkentin JD, Williams JA. Mol Biol Cell. 2008 Jan;19(1):198-206.
- Regulator of calcineurin 1 controls growth plasticity of adult pancreas. Gurda GT, Crozier SJ, Ji B, Ernst SA, Logsdon CD, Rothermel BA, Williams JA. Gastroenterology. 2010 Aug;139(2):609-19