Description (including epitope tag if present): 

NFAT-luciferase reporter (9 multimerized NFAT binding sites upstream of a minimal TATA-containing promoter fused to luciferase) was cloned into the multiple cloning site of pAC-CMV-pLpA construct. The resulting plasmid was cotransfected into HEK293 cells with plasmid pJM17 which contains the Ad5 genome.  


  • The adenoviral vector is now commercially available through Seven Hills Bioreagents:
  • How used: 

    Optimized and performed in isolated, primary mouse acinar cells as well as secondary cell lines including

    acinar-derived AR42J, 266-6 and non-acinar HEK293 and NIH 3T3.  Please refer to methods section in pancreapedia "Quantitative assays of transcriptional activity:  NFAT-Luciferase reporter".

    Publications describing the vector and usage: 

    1. Cholecystokinin activates pancreatic calcineurin-NFAT signaling in vitro and in vivo. Gurda GT, Guo L, Lee SH, Molkentin JD, Williams JAMol Biol Cell. 2008 Jan;19(1):198-206.                                      
    2. Regulator of calcineurin 1 controls growth plasticity of adult pancreas.  Gurda GT, Crozier SJ, Ji B, Ernst SA, Logsdon CD, Rothermel BA, Williams JAGastroenterology. 2010 Aug;139(2):609-19