Source:
Santa Cruz Biotechnology, Inc. sc-654 AC; BD Transduction Laboratories, 610297 (old #N30020)
Description:
Rabbit polyclonal agarose conjugate against human NOS3 (sc-654 AC). Mouse monoclonal against human eNOS (610297). The products are affinity purified immunoglobulin fractions.
How used:
Immunoprecipitation of 0.25 mg lysate protein overnight with 0.025 ml agarose conjugate in 1 ml lysis buffer. Western Blot 1:500 in 5% BSA 1 hour, secondary GE healthcare anti-mouse-HRP (NXA931) 1:10,000 in 5% milk 1 hour. Approximate molecular weight 140 kDa.
Publications describing the antibody and usage:
1. Endothelial nitric oxide synthase is protective in the initiation of caerulein-induced acute pancreatitis in mice. DiMagno, M. J., J. A. Williams, et al. (2004). Am J Physiol Gastrointest Liver Physiol. 2004 Jul;287(1):G80-87. PMID: 14962849