Chymotrypsin

Source: 

Fitzgerald Industries CR1095R

Description: 

Rabbit polyclonal raised against chymotrypsin from human pancreas. Reacts with rat and mouse chymotrypsin

How used: 

western blot 1:4000 in 5% BSA overnight, secondary Santa Cruz anti-rabbit-HRP (sc-2004) 1:500 in 5% milke 1 hour. Approximate molecular weigh 34kDa

Publications describing the antibody and usage: 

  1. CCK-independent mTORC1 activation during dietary protein-induced exocrine pancreas growth.  Crozier SJ, Sans MD, Wang JY, Lentz SI, Ernst SA, Williams JA.  Am J Physiol Gastrointest Liver Physiol. 2010 Nov;299(5):G1154-63. PMID: 20798356                                                                                                                                                       
  2. Molecular mechanisms of pancreatic dysfunction induced by protein malnutrition.  Crozier SJ, D'Alecy LG, Ernst SA, Ginsburg LE, Williams JA.  Gastroenterology. 2009 Sep;137(3):1093-101, 1101.e1-3. Epub 2009 May 7. PMID:  19427311