Amylase polyclonal


Sigma A-8273


Rabbit polyclonal against human salivary α-amylase. The product is a semipurified immunoglobulin fraction.

How used: 

Western Blot 1:20000  in 5% BSA overnight, secondary GE healthcare anti-mouse-HRP (NXA931) 1:5000 in 5% milk 1 hour. Approximate molecular weight 60kDa.  The antibody also can be used for immunohistochemistry at a dilution of 1:500 - 1:2000.


Publications describing the antibody and usage: 

  1. CCK-independent mTORC1 activation during dietary protein-induced exocrine pancreas growth.  Crozier SJ, Sans MD, Wang JY, Lentz SI, Ernst SA, Williams JAAm J Physiol Gastrointest Liver Physiol. 2010 Nov;299(5):G1154-63. PMID:  20798356
  2. Rab3D localizes to zymogen granules in rat pancreatic acini.  Ohnishi H, Ernst SA, Wys N, McNiven M, Williams JA.  Am J Physiol: Gastrointestinal Liver Physiology 271:G531-G538, 1996.  PMID: 8843780