Source:
Sigma A-8273
Description:
Rabbit polyclonal against human salivary α-amylase. The product is a semipurified immunoglobulin fraction.
How used:
Western Blot 1:20000 in 5% BSA overnight, secondary GE healthcare anti-mouse-HRP (NXA931) 1:5000 in 5% milk 1 hour. Approximate molecular weight 60kDa. The antibody also can be used for immunohistochemistry at a dilution of 1:500 - 1:2000.
Publications describing the antibody and usage:
- CCK-independent mTORC1 activation during dietary protein-induced exocrine pancreas growth. Crozier SJ, Sans MD, Wang JY, Lentz SI, Ernst SA, Williams JA. Am J Physiol Gastrointest Liver Physiol. 2010 Nov;299(5):G1154-63. PMID: 20798356
- Rab3D localizes to zymogen granules in rat pancreatic acini. Ohnishi H, Ernst SA, Wys N, McNiven M, Williams JA. Am J Physiol: Gastrointestinal Liver Physiology 271:G531-G538, 1996. PMID: 8843780