Gene Symbols: ITPR1, ITPR2, ITPR3

Other Names:  IP₃R1, IP₃R2, IP₃R3

1. General: The role of InsP₃R in pancreatic acinar and other cells

The classical pathway linking stimulation by neurotransmitters and hormones to changes in phosphoinositide metabolism and the subsequent InsP₃ induced Ca²⁺ release was in large part documented by work carried out in exocrine cells, in particular, acinar cells isolated from the exocrine pancreas (62). In seminal work using permeabilized rat pancreatic acini, Streb and colleagues demonstrated that the addition of InsP₃ resulted in Ca²⁺ release from a non-mitochondrial Ca²⁺ store (54). This store was later identified to be the endoplasmic reticulum (ER) through experiments where sub-cellular fractions from pancreatic cells were isolated and exposed to InsP₃ (6, 53). Ca²⁺ release is rapid, occurring as quickly as 1s following secretagogue stimulation of acinar cells. Ca²⁺ release is also isomer specific, with other structurally related inositol phosphates shown to be far less efficacious in releasing Ca²⁺(54). The target protein for InsP₃ binding was later identified as the inositol 1,4,5-trisphosphate receptor (InsP₃R) (56). Strikingly, the secretion of digestive enzymes from pancreatic acinar cells has been shown to be entirely dependent on the activation of InsP₃Rs and the resulting elevation in intracellular [Ca²⁺] (22). The receptor is encoded by 3 distinct genes in mammalian cells (ITPR1, ITPR2, ITPR3) that generate 3 monomeric isoforms (R1, R2, R3 respectively) that share 60-70% sequence homology (47).

Originally purified and cloned from rat cerebellum, the full length InsP₃R forms a tetrameric cation selective channel in vivo (40). The three isoforms of InsP₃R exhibit overlapping patterns of expression, with most cells and tissues expressing more than one isoform (63). Western blotting and quantitative PCR has revealed that there is relatively equal expression of the R2 and R3 isoforms in pancreas, with R1 only constituting ~3% of the total InsP₃R (63). The importance ofR2 and R3 isoforms to pancreatic Ca²⁺ signaling is most evident in studies employing InsP₃R knockout (KO) mice (22). Although single R2 or R3 KO mice were found to have no observable ill effects or phenotypic alterations, double R2/R3 KO mice typically gained less body weight post-natally compared to single KO or WT mice. Furthermore, double KO mice tended to lose weight rapidly post weaning and typically died 4 weeks after birth. These observations were attributed to the key role the R2 and R3 isoforms play in the secretion of saliva and exocytosis of digestive enzymes from exocrine glands and the resulting inability of double KO mice to swallow or properly digest dry adult food. Indeed, dou ble KO mice were rescued and reached body weights comparable to WT when a fed pre-digested wet mash diet or an elemental diet. Pancreatic acinar cells from R2/R3 double KO mice were also found to accumulate zymogen granules and failed to secrete zymogen granules on stimulation with secretagogues. The study also highlights the inability of R1 to rescue double R2/R3 KO mice (22).

The fidelity and specificity of the Ca²⁺ signal required for exocytosis is thought to be largely determined by the differential expression, localization and modulation exhibited by the 3 InsP₃R isoforms (21). For the most part , InsP₃Rs are predominantly localized to the ER, although the golgi, nucleus, plasma membrane, peroxisomes and endolysosomal vesicles have also been reported to express small levels of InsP₃Rs. Pancreatic acinar cells are highly polarized, both functionally and morphologically and InsP₃Rs are predominantly expressed in ER extensions in the apical regions juxtaposed to the acinar lumen (Figure 1) (34, 36, 42, 69). Using fluorescence imaging techniques, studies have shown that stimulating InsP₃ generation in these cells results in the initiation of Ca²⁺ signals in the apical regions, followed by the propogation basally of a Ca²⁺ wave that is facilitated by peripheral InsP₃Rs. Stimulation of basal secretagogue receptors as well as uncaging InsP₃ in various regions of the cell also confirm that Ca²⁺ signals initiate in apical regions of the pancreatic acinar cell (5, 20, 31, 58, 65).

Figure 1: Localization of InsP₃Rs in pancreatic acinar cells. Immunofluorescence localizations in pancreatic lobules of InsP₃Rs by confocal microscopy demonstrate the localization of InsP3R1, R2, R3 and amylase (A,B,C,D respectively) respectively. All 3 isoforms are predominantly localized to the apical pole of acinar cells directly abutting the plasma membrane (arrows in panels A-C). R1 and R3 also appeared to localize to perinuclear structures. (Scale bar = 10 μm). (69)

2. Structural features of the InsP₃R

Based on the sequence homology, all three isoforms of InsP₃R are predicted to have the same general structural domains (12). Specifically, the InsP₃R monomer is conventionally divided into 3 functional domains: an N-terminal ligand binding domain (LBD), a coupling and modulatory domain and a C-terminal transmembrane domain (TMD) that contains the channel (21) (Figure 2A). To date, the majority of structural insights were from the investigations of R1, including the recent structures of R1 revealed by cryo-EM (Figure 2B and 2C) andx-ray crystallography (26).Therefore, in this review, the locations of common functional modules in InsP₃R, which are also present in R2 and R3 at homologous regions, are described using the amino acid sequence of R1. InsP₃ binding is mediated by the ‘core’ ligand binding domain, which constitutes amino acids (AA) 224-578 of the LBD. This region contains 10 conserved positively charged arginine and lysine residues (3 critical, R265, K508, R511) that are thought to allosterically coordinate the negatively charged PO₄^3- groups of InsP₃ in a binding pocket (67). By using concatenated subunits (1, 18), our group has recently reported that InsP₃ molecules must be bound to each monomer of the four subunits to open the channel and activate InsP₃R (2). This gating mechanism ensures the fidelity of InsP₃ induced calcium release and may provide mechanistic insight into diseases where mutations have an impact on InsP₃ binding to the receptor. The three isoforms have differing InsP₃ binding affinities that are regulated by the first 223 AA of the LBD, termed the suppressor domain (SD). Specifically, competitive InsP₃ binding assays using GST fusion constructs encoding AA 1-604 demonstrate that R2 has an 11-fold greater affinity (Kd) for InsP₃ compared to R3 (14 nM vs. 163 nM), with R1 having an intermediate affinity (49.5 nM) (29). Similar studies in pancreatic acini revealed a Kd of 6 nM (24), comparable to that of R2, while permeabilized Ca²⁺ release assays in acinar cells demonstrated an EC₅₀ for InsP₃ of 0.8 µM (45) (Figure 3).Deletion of the suppressor domain (SD) results in the loss of distinct InsP₃ affinities between the isoforms and a 10-100 fold increase in InsP₃ affinity (29). Despite an increased InsP₃ binding affinity, deletion of the SD also results in the loss of channel activity, indicating that the SD is required for inducing InsP₃R activation and Ca²⁺ release. The pivotal role of SD for InsP₃R activation was also strongly supported by Cryo-EM structure of homotetrameric R1 solved at near-atomic resolution (19). The Serysheva group showed that the SD domain forms inter-subunit interactions with ARM2, ARM3, β-TF2 and CTD domains of adjacent subunits, which may underlie a direct functional coupling between the N- and C- terminus of the receptor (19). Based on hydropathy plots, the TMD is similar in structure to that of RyRs and voltage gated K⁺, Na⁺ and Ca²⁺ channels and constitutes 6 putative transmembrane regions (TM1-6) (16). The TMD is responsible for the ER targeting (44, 46) and for the oligomerization of the InsP₃R into tetramers, which occurs co-translationally (30). Lastly, TM5 and 6 forms the pore through which Ca²⁺ is conducted (48). The loop between TM5 and 6 contains a selectivity filter (GVGD; similar to the super family of cation selective channels) that provides some degree of cation selectivity to the InsP₃R (21). However, it is poorly Ca²⁺ selective and allows conduction of monovalent cations (Ca²⁺:K⁺ = 6:1). In fact, it is believed that  “functional” Ca²⁺ selectivity of InsP₃R is primarily determined by virtue of SERCA being a highly selective Ca²⁺ pump and Ca²⁺ being by the far the most abundant cation in the ER.  To “gate” and open the channel, . The CT tail (last 160 AA) and the large (1700 AA) but less conserved modulatory domain contains putative binding sites for the numerous modulators of InsP₃R activity (68). These modulators, which include Ca²⁺, ATP and PKA, all contribute in distinct ways to the differential Ca²⁺ release profiles encoded by the 3 isoforms.

Figure 2: Illustration of R1 depicting location of suppressor domain, ligand binding core, regulation and coupling domain and channel domain (A). Structure of R1 revealed by Cryo-electron microscopy at near atomic resolution of 4.7 A (B and C). (B) is a side view along membrane plane of a R1 monomer. (C) is a tetrameric R1 viewed from cytosol. Each monomer of the tetramer are color-coded. Figures are adapted from (19)

Figure 3: (A) Permeabilized cell Ca²⁺ release assay in acinar cells. Isolated pancreatic acini were loaded with furaptra prior to permeabilization with β-escin. ER is loaded through SERCA upon application of Ca²⁺ containing buffer containing MgCl₂, and ATP, as indicated by the increase in fluorescence ratio. Removal of MgCl₂ deactivates SERCA and (B) addition of varying [InsP₃] releases from stores as indicated by the decrease in fluorescence ratio. (C) Concentration response analysis for InsP_3. EC₅₀ for InsP₃ is 0.8 µM (10)

3. Modulation of the InsP₃R

Cytosolic Ca²⁺ is the most important regulator of InsP₃Rs, modulating activity in a biphasic manner (14). Numerous putative Ca²⁺ binding sites have been identified, and Ca²⁺ has been shown to induce dramatic conformational changes in R1 (3, 4, 25, 49, 50). For the most part, studies have shown that in the presence of InsP₃, low to optimal [Ca²⁺] (300 nM) stimulates channel activity while higher cytosolic [Ca²⁺] inhibits it (14). To explain this biphasic regulation, Foskett and collaborators have proposed a model in which InsP₃R activity is regulated by two distinct Ca²⁺ binding sites: a stimulatory and an inhibitory site. Under resting conditions, the inhibitory site has a higher affinity for Ca²⁺ than the stimulatory site. Accordingly, this site is occupied at resting conditions and inhibits InsP₃R activity. The binding of InsP₃ decreases the inhibitory sites’ affinity for Ca²⁺, thereby allowing Ca²⁺ to bind to the stimulatory site and positively regulating channel activity. The downstroke of the biphasic curve is the result of Ca²⁺ binding to the inhibitory sites due to cytosolic [Ca²⁺] being elevated beyond 300 nM (57). In effect, they suggest that Ca²⁺ is only an essentially InsP₃R co-agonist, with InsP₃s sole role being simply to modulate Ca²⁺ sensitivities (37).

Cytosolic ATP has also been shown to differentially modulate the activity of all 3 isoforms in an allosteric manner. This mode of regulation is thought to link the metabolic status of the cell to the Ca²⁺ release. Specifically, studies show that R2 is only modulated by ATP at sub-maximal [InsP₃] while R1 and R3 activity is affected irrespective of [InsP₃], implying that ATP is required for maximal activity of R1 and R3 (8, 9).  Each isoform also differs in its affinity for ATP, with R2 having 3 fold and 10 fold higher affinities than R1 and R3, respectively (8). ATP binding was originally believed to occur at Walker A like motifs (G-X-G-X-X-G) that exist in each isoform. One such motif, called ATPB, is conserved across all 3 isoforms. Additionally, R1 contains 2 other sites: ATPA and ATPC, the latter only being found in the S2- variant which is expressed in peripheral tissues (59). Recently, mutagenesis studies have demonstrated that only the ATPB site in R2 is important in mediating the modulatory effects of ATP (45) and this regulation is important for defining the sensitivity of Ca²⁺ release in pancreatic acinar cells (24). In contrast, the ATPB sites in R1 and R3, in addition to the ATPA and ATPC sites in R1, play no role in modulating InsP₃R activity through ATP. 

Further, another level of receptor regulation results from the subunit composition of InsP₃R. Most cells express more than one isoform of InsP₃R; as a result, different isoforms of InsP₃R display overlapping expression patterns (63). Extensive studies with various experimental techniques, including cross-linking (15, 43), immunostaining (27, 33, 34, 69), and co-immunoprecipitation (1, 64), have consistently demonstrated that InsP₃R can be hetero-tetrameric channels formed from at least two isoforms of InsP₃R. An obvious question is what is the contribution of each subunit in a hetero-tetramer? Our lab showed that  an individual subtype can either contribute equally, or dictate the Ca²⁺ release properties of the hetero-tetrameric InsP₃R, depending on the presence and the concentrations of key regulators (ATP and Ca²⁺) (17). In the context of the pancreas, R2 and R3 are the predominant isoforms and form hetero-tetramers accounting for the major proportion of the InsP₃R (63). A hetero-tetramer containing two R2 and two R3 is predicted to exhibit blended channel activities under optimal conditions; in contrast, R2 in the same hetero-tetramer can dictate the channel activities under suboptimal conditions (17).

Region-specific proteolysis is a novel form of regulation for all three isoforms of InsP₃R. R1 has been well demonstrated to be a substrate for the intracellular cysteine proteases, caspase and calpain (28, 32). However, the functional consequences of receptor fragmentation are a subject of considerable debate. Recent evidence strongly suggests that region-specific proteolysis modifies R1 channel regulation (51, 60, 61). Specifically, stimulation of caspase or calpain fragmented R1 results in Ca²⁺ signals with distinct temporal characteristics and can activate alternative signaling pathways (51, 61). Further studies are necessary to guage if R2 and R3 are subject to similar regulation.

Lastly, protein kinase A (PKA) mediated phosphorylation has been shown to directly increase InsP₃R Ca²⁺ flux and single channel activity of R1 and R2 albeit by phosphorylation at different residues. Specifically, studies show that PKA mediated phosphorylation at S1598 and S1755 on R1 and S937 on R2 significantly enhance Ca²⁺ release and single channel activity (7, 11, 68). Conversely, no effects on Ca²⁺ release through R3 have been observed after PKA stimulation, despite evidence that R3 is phosphorylated by PKA at 3 sites in vivo (52, 68). Thus far, no single channel studies have been performed on R3 to rule out any PKA mediated effects on channel activity.

InsP₃Rs are also regulated and bound by numerous other kinases and accessory proteins, including cGMP protein kinase (PKG), Akt kinase, FK506 binding protein, calmodulin, CaBP, IRBIT, Bcl-2/XL, cytochrome C, RACK and Erp44 (21).

4. Tools available to study the InsP₃R

A. Antibodies

A multitude of commercially available isoform specific antibodies can be utilized to probe for each isoform of the InsP₃R. Additionally, there are antibodies that detect all 3 isoforms

• InsP₃R1 Rabbit polyclonal (H-80) from Santa Cruz (sc-28614); epitope corresponding to AA 1894-1973 in the cytosolic modulatory domain
• InsP₃R1, UC Davis/NIH NeuroMab Facility, clone L24/18; epitope against AA 2680-2749 in C-terminus
• InsP₃R3 Goat polyclonal (C-20) from Santa Cruz (sc-7277); epitope corresponding to C-terminus
• InsP₃R3 purified mouse monoclonal from BD Transduction Laboratories (Cat: 610313)
• InsP₃R1/2/3 Rabbit polyclonal (H-300) from Santa Cruz (sc-28613); epitope corresponding to AA 2402-2701 in C-terminus of InsP₃R-2 (human)

B. cDNA

Accession number:

• InsP₃R1: rat, 55925609
• InsP₃R2: mouse, 60593031
• InsP₃R3: rat, 6981109

C. Cell Lines

• SHSYFY Neuroblastoma: 99% InsP₃R1 (63)
• AR42J Rat pancreatoma: 86% InsP₃R2; 12% InsP₃R1; 2% InsP₃R3 (63)
• RINM5F Mouse insulinoma: 96% InsP₃R3; 4% InsP₃R1(63)

DT40 triple knockout cell lines: Kurosaki and colleagues have generated a DT40 chicken B lymphocyte cell line with the endogenous InsP₃Rs stably knocked out (55). These triple knockout cells can subsequently be stably transfected with constructs encoding individual mammalian InsP₃R isoforms, allowing the study of activity and regulation of each isoform in isolation. These cell lines are available on request from our laboratory.

HEK triple knockout cell line: David Yule and colleagues have generated HEK triple knockout cell line with the endogenous InsP₃Rs stably knockout (2). In addition to DT40 triple knockout cell line, HEK triple knockout cell line provides an alternative mammalian InsP₃R null background system for research.

D. Mouse Lines

InsP₃R-1 knockout mice (39)

InsP₃R-2 (35) and R-3 single and double knockout mice (22)

E. Agonists/Antagonists/

Agonists

Commercially available InsP₃: D-myo-inositol 1,4,5-trisphoshate hexapotassium salt; Enzo Life Sciences (cat: ALX-307-00)
Cells are often stimulated with Gq coupled GPCR agonists to stimulate InsP₃ production. Agonists include acetylcholine, trypsin and cholecystokinin.

Antagonists

Heparin (66); Caffeine (13); 2-Aminoethoxydiphenyl borate (2-APB) (38, 41); Xestospongin (23)

F. Techniques used to study InsP₃R function

See review by Betzenhauser MJ., Wagner LE., Won JW and Yule DI. (2008) Studying isoform-specific inositol 1,4,5-trisphosphate receptor function and regulation. Methods 46, pp: 177-182. (10)

Betzenhauser, Matthew J., Won, Jong Hak, Park, Hyungseo and Yule, David I. (2011). Measurement of Intracellular Calcium Concentration in Pancreatic Acini. The Pancreapedia: Exocrine Pancreas Knowledge Base, DOI:10.3998/panc.2011.34

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